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Staphylococcus aureus is known to express the single plasminogen activator staphylokinase and a few plasminogen receptors, such as Sbi, Ebh and α-enolase, on its surface [13, 22, 32, 33].We next wanted to examine whether S. aureus NCTC expresses putative Plg receptors in addition to the well-known ones. To explore that, we screened the polypeptides of the FTP library for their binding. Xkiss - casting in Keulen part 4. Made in Holland. Buy this video for 75 TK. The hot sexual adventures of French and Walloon part 3. Made in Holland. Buy this video for 75 TK. The French and the Belgians greet you part 1. Made in Holland. Buy this video for 75 TK. Xkiss - casting in Keulen . Alexander in legend and romance. Alexander the Great was an immensely popular figure in the classical and post-classical cultures of the Mediterranean and Middle hekiga3d.com immediately after his death in BC a body of legend began to accumulate about his exploits and life which, over the centuries, became increasingly fantastic as well as allegorical.

Pinho MG, de Lencastre H, Tomasz A. Cloning, characterization, and inactivation of the gene pbpC, encoding penicillin-binding protein 3 of Staphylococcus aureus.

Derbise A, Song YP, Parikh S, Fischetti VA, Pancholi V. Role of the C-terminal lysine residues of streptococcal surface enolase in Glu- and Lys-plasminogen-binding activities of group A streptococci.

Wistedt AC, Ringdahl U, Müller-Esterl WSU. Identification of a plasminogen-binding motif in PAM, a bacterial surface protein.

Sanderson-Smith ML, Dowton M, Ranson M, Walker MJ. The plasminogen-binding group A streptococcal M protein-related protein Prp binds plasminogen via arginine and histidine residues.

Schenone MM, Warder SE, Martin JA, Prorok M, Castellino FJ. An internal histidine residue from the bacterial surface protein, PAM, mediates its binding to the kringle-2 domain of human plasminogen.

J Pept Res. Griffin JD, Ellman L. Epsilon-aminocaproic acid EACA. Semin Thromb Hemost. Silva MMCG, Thelwell C, Williams SC, Longstaff C.

Regulation of fibrinolysis by C-terminal lysines operates through plasminogen and plasmin but not tissue-type plasminogen activator.

Bergmann S, Wild D, Diekmann O, Frank R, Bracht D, Chhatwal GS, Hammerschmidt S. Mattler LE, Bang NU. Serine protease specificity for peptide chromogenic substrates.

Muthukrishnan G, Quinn GA, Lamers RP, Diaz C, Cole AL, Chen S, Cole AM. Exoproteome of Staphylococcus aureus reveals putative determinants of nasal carriage.

J Proteome Res. Surfaceome and exoproteome of a clinical sequence type methicillin resistant Staphylococcus aureus strain.

Biochem Biophy Rep. Google Scholar. Gurung M, Moon DC, Choi CW, Lee JH, Bae YC, Kim J, Lee YC, Seol SY, Cho DT, Kim SI, Lee JC. Staphylococcus aureus produces membrane-derived vesicles that induce host cell death.

Papasergi S, Galbo R, Lanza-Cariccio V, Domina M, Signorino G, Biondo C, Pernice I, Poyart C, Trieu-Cuot P, Teti G, Beninati C. Analysis of the Streptococcus agalactiae exoproteome.

J Proteomics. Hensbergen PJ, Klychnikov OI, Bakker D, van Winden VJC, Ras N, Kemp AC, Cordfunke RA, Dragan I, Deelder A, Kuijper EJ, Corver J, Drijfhout JW, van Leeuwen HC.

A novel secreted metalloprotease CD from Clostridium difficile cleaves specific proline sequences in LPXTG cell surface proteins.

Mol Cell Proteomics. Pacheco LG, Slade SE, Seyffert N, Santos AR, Castro TL, Silva WM, Santos AV, Santos SG, Farias LM, Carvalho MA, Pimenta AM, Meyer R, Silva A, Scrivens JH, Oliveira SC, Miyoshi A, Dowson CG, Azevedo V.

A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis. Henderson B, Martin AC. Protein moonlighting: a new factor in biology and medicine.

Biochem Soc Trans. Wertheim HF, Vos MC, Ott A, van Belkum A, Voss A, Kluytmans JA, van Keulen PH, Vandenbroucke-Grauls CM, Meester MH, Verbrugh HA.

Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers. Michiels B, Appelen L, Franck B, den Heijer CDJ, Bartholomeeusen S, Coenen S.

Staphylococcus aureus , including meticillin-resistant Staphylococcus aureus , among general practitioners and their patients: a cross-sectional study.

Biofilm matrix exoproteins induce a protective immune response against Staphylococcus aureus biofilm infection. Download references.

RK participated in the design of the study and interpretation of data, carried out the protein purifications and functional analysis with purified proteins in time-resolved fluorometry and ELISA Figs.

TO carried out the adherence assays with bacterial cells Fig. VK participated in development of the bacterial adhesion assay, the time-resolved fluorometry and purified the control protein ST1 Eno of L.

She also participated in writing of the manuscript. RV participated in planning and supervision of the bacterial adhesion assay, and participated in writing of the manuscript.

BWW directed the study, had the main responsibility for data interpretation and manuscript writing. All authors read and approved the final manuscript.

Sandra Heinzelmann, Jukka-Pekka Palomäki, Laura Huuskonen and Anna Jalava are acknowledged for skilled technical assistance.

The datasets supporting the conclusions of this article are included within the article and its additional files. Present address: Thermo Fisher Scientific, Ratastie 2, , Vantaa, Finland.

Present address: Orion Diagnostica, Koivu-Mankkaan tie 6, , Espoo, Finland. At the beginning of the session we will clarify your experience in BDSM and agree your personal setting.

If you are looking for something a little different or are watching the pennies then these options might just throw up some tempting treats:.

If you are pushed for time, you can even visit a drive-in brothel! Situated an hour from the Dutch border and between Bonn and Dusseldorf, Cologne is extremely well-served by its own sex scene but is also close to plenty of other opportunities if you have a car.

There are dozens of brothels around Cologne with some offering a unique experience such as Pascha, the largest bordello in Europe, whilst others have a more boutique edge.

The building covers a total space of m 2 and offers a wide range of entertainment over 12 floors.

The nightclub at Pascha offers strip shows, lap dances and more. Image via website. There are around prostitutes working in the building, all operating as independent sex workers.

Pascha is definitely an experience that every man should have under his belt and is well worth spending some time here. It is worth trying Pascha out during a busy evening to really get an eyeful but you can also get some bargains during quieter periods.

Haus 80 is well situated in the north of the city quite close to the Hansaring railway junction. However, faces change very frequently and there are occasions when punters can get a poor experience.

There are quite a few negative reviews for the place, mainly due to the prices but this is not a sauna club; it is a discerning bordello that keeps rates high to match standards.

As well as in-house services they also offer quality escorts for dinner dates, business lunches and private travel. All ladies are delivered by a luxury chauffeur and offer excellent companionship.

Rooms are themed with Africa, Rome, Asia and Egypt all being offered up…though somewhat tired looking in places.

This is a private house brothel and not an FKK sauna or nightclub so there is nothing else here to enjoy but the ladies.

Speaking of whom, there are a dozen or so women working here but usually no more than a handful to select from on any given rota. A selection of the girls available at Cat Exclusive.

There is a good selection of girls on offer and, though much smaller than Pascha, offers a discreet boutique approach to choosing a prostitute.

There are several well-appointed rooms, each well designed for comfort and with a classy hotel style. Some have spa baths whilst others simply have shower facilities.

The main resident, Soi, is a Thai girl in her twenties and offers both in-calls and out-calls. Image via Romabad. There are less sex cinemas in Cologne than there were a few decades ago and many of those that re left are mainly used by gay men.

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The Muslim geographer Yaqut al-Hamawi further confirmed the same view in a number of places in his book on geography; for instance under the heading "Khazar" Caspian he writes:.

This territory adjoins the Wall of Dhul-Qarnain just behind Bab-ul-Abwab, which is also called Derbent.

The Caliph Harun al-Rashid — AD even spent some time living in Derbent. Not all Muslim travellers and scholars, however, associated Dhul-Qarnayn's wall with the Caspian Gates of Derbent.

For example, the Muslim explorer Ibn Battuta — AD travelled to China on order of the Sultan of Delhi , Muhammad bin Tughluq and he comments in his travel log that "Between it [the city of Zaitun in Fujian ] and the rampart of Yajuj and Majuj [Gog and Magog] is sixty days' travel.

In the Quran, it is none other than the Gog and Magog people whom Dhul-Qarnayn has enclosed behind a wall, preventing them from invading the Earth.

In Islamic eschatology , before the Day of Judgement Gog and Magog will destroy this gate, allowing them to ravage the Earth, as it is described in the Quran:.

In the Syriac Christian legends, Alexander the Great encloses the Gog and Magog horde behind a mighty gate between two mountains, preventing Gog and Magog from invading the Earth.

In addition, it is written in the Christian legend that in the end times God will cause the Gate of Gog and Magog to be destroyed, allowing the Gog and Magog horde to ravage the Earth;.

The Lord spake by the hand of the angel, [saying] The gate of the north shall be opened on the day of the end of the world, and on that day shall evil go forth on the wicked The earth shall quake and this door [gate] which thou [Alexander] hast made be opened And the nations that is within this gate shall be roused up, and also the host of Agog and the peoples of Magog [Gog and Magog] shall be gathered together.

These peoples, the fiercest of all creatures. The Christian Syriac legend describes a flat Earth orbited by the sun and surrounded by the Paropamisadae Hindu Kush mountains.

The Paropamisadae mountains are in turn surrounded by a narrow tract of land which is followed by a treacherous Ocean sea called Okeyanos.

It is within this tract of land between the Paropamisadae mountains and Okeyanos that Alexander encloses Gog and Magog, so that they could not cross the mountains and invade the Earth.

The legend describes "the old wise men" explaining this geography and cosmology of the Earth to Alexander, and then Alexander setting out to enclose Gog and Magog behind a mighty gate between a narrow passage at the end of the flat Earth:.

The old men say, "Look, my lord the king, and see a wonder, this mountain which God has set as a great boundary.

And he had it in his mind to make there a great gate. His mind was full of spiritual thoughts, while taking advice from the old men, the dwellers in the land.

He looked at the mountain which encircled the whole world The king said, "Where have the hosts [of Gog and Magog] come forth to plunder the land and all the world from of old?

Flat Earth beliefs in the Early Christian Church varied and the Fathers of the Church shared different approaches.

Those of them who were more close to Aristotle and Plato 's visions, like Origen , shared peacefully the belief in a spherical Earth.

A second tradition, including St Basil and St Augustine , accepted the idea of the round Earth and the radial gravity, but in a critical way.

In particular they pointed out a number of doubts about the antipodes and the physical reasons of the radial gravity. However, a flat Earth approach was more or less shared by all the Fathers coming from the Syriac area, who were more inclined to follow the letter of the Old Testament.

Diodore of Tarsus? In the Christian Alexander romance literature, Gog and Magog were sometimes associated with the Khazars , a Turkic people who lived near the Caspian Sea.

In his 9th century work Expositio in Matthaeum Evangelistam , the Benedictine monk Christian of Stavelot refers to the Khazars as Hunnic descendants of Gog and Magog, and says they are "circumcised and observing all [the laws of] Judaism" ; [55] the Khazars were a Central Asian people with a long association with Judaism.

A Georgian tradition, echoed in a chronicle, also identifies the Khazars with Gog and Magog, stating they are "wild men with hideous faces and the manners of wild beasts, eaters of blood.

Early Muslim scholars writing about Dhul-Qarnayn also associated Gog and Magog with the Khazars. Ibn Kathir — CE , the famous commentator of the Quran, identified Gog and Magog with the Khazars who lived between the Black and Caspian Sea in his work Al-Bidayah wa al-Nihayah The Beginning and the End.

Thus Muslim scholars associated the Khazars with Dhul-Qarnayn just as the Christian legends associated the Khazars with Alexander the Great.

A peculiar aspect of the story of Dhul-Qarnayn, in the Quran, is that it describes Dhul-Qarnayn travelling to "the rising place of the Sun" and the "setting place of the Sun," where he saw the Sun sets into a murky or boiling spring of water or mud.

Dhul-Qarnayn also finds a people living by the "rising place of the Sun," and finds that these people somehow have "no shelter.

In his commentary of the Quran, Ibn Kathir — CE explains that verse is referring to the furthest point that could be travelled west:.

Until, when he reached the setting place of the sun, means, he followed a route until he reached the furthest point that could be reached in the direction of the sun's setting, which is the west of the earth.

As for the idea of his reaching the place in the sky where the sun sets, this is something impossible, and the tales told by storytellers that he traveled so far to the west that the sun set behind him are not true at all.

Most of these stories come from the myths of the People of the Book [Jews and Christians] and the fabrications and lies of their heretics.

In this commentary Ibn Kathir differentiates between the end of the Earth and the supposed "place in the sky" where the sun sets the "resting place" of the sun.

Ibn Kathir contends that Dhul-Qarnayn did reach the farthest place that could be travelled west but not the "resting place" of the sun and he goes on to mention that the People of the Book Jews and Christians tell myths about Dhul-Qarnayn travelling so far beyond the end of the Earth that the sun was "behind him.

A similar theme is elaborated upon in several places in the Islamic hadith literature, in Sahih al-Bukhari and Sahih Muslim :.

It is narrated They replied: Allah and His Apostle know best. He the Holy Prophet observed: Verily it the sun glides till it reaches its resting place under the Throne [of Allah].

Then it falls prostrate and remains there until it is asked: Rise up and go to the place whence you came, and it goes back and continues emerging out from its rising place The setting place of the sun is also commented on by Al-Tabari AD and Al-Qurtubi — AD and, like Ibn Kathir , they showed some reservations towards the literal idea of the sun setting in a muddy spring but held to the basic theme of Dhul-Qarnayn reaching the ends of the Earth.

That the Earth must be spherical was known since at least the time of Pythagoras — BC , but this knowledge did not reach ancient folklore such as the Alexander romance where Alexander travels to the ends of a flat Earth.

It is notable that, unlike the Babylonians, Greeks, and Indians, the pre-Islamic Arabs had no scientific astronomy. Their knowledge of astronomy was limited to measuring time based on empirical observations of the "rising and setting" of the sun, moon, and particular stars.

This area of astronomical study was known as anwa and continued to be developed after Islamization by the Arabs.

The work is significant as it introduced the Ptolemaic system into Islamic sciences the Ptolemaic system was ultimately replaced by the Copernican system during the Scientific Revolution in Europe.

Rendition of Homer 's view of the world prior to BC. The Homeric conception of the world involved a flat, circular Earth, surrounded by mountains and by Oceanus , the world-ocean of classical antiquity , considered to be an enormous river encircling the world.

The Sun emerges from underneath the Earth, travelling along the fixed dome of the sky, and is shown rising from Oceanus. The place of his [the Sun's] rising is over the sea, and the people who dwell there, when he is about to rise, flee away and hide themselves in the sea, that they be not burnt by his rays; and he passes through the midst of heaven to the place where he enters the window of heaven; and wherever he passes there are terrible mountains, and those who dwell there have caves hollowed out in the rocks, and as soon as they see the Sun passing [over them], men and birds flee away from before him and hide in the caves And when the Sun enters the window of heaven, he [it] straight away bows down and makes obeisance before God his Creator; and he travels and descends the whole night through the heavens, until at length he finds himself where he [the Sun] rises So the whole camp mounted, and Alexander and his troops went up between the fetid sea and the bright sea to the place where the Sun enters the window of heaven; for the Sun is the servant of the Lord, and neither by night nor by day does he cease from his travelling.

The Christian legend is much more detailed than the Quran's version and elaborates at length about the cosmology of the Earth that is implied by the story:.

He [Alexander] said to them [the nobles]: "This thought has arisen in my mind, and I am wondering what is the extent of the earth, and how high the heavens are He explained that he had been instructed to "take the herb from the mouth of the devil and return it.

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